Amino acid-specific ADP-ribosylation. Sensitivity to hydroxylamine of [cysteine(ADP-ribose)]protein and [arginine(ADP-ribose)]protein linkages.
نویسندگان
چکیده
منابع مشابه
Amino Acid-specific ADP-ribosylation STABILITY OF THE REACTION PRODUCTS OF AN NADzARGININE ADP-RIBOSYLTRANSFERASE TO HYDROXYLAMINE AND HYDROXIDE*
[adenine-U-14C]ADP-ribose-agmatine and [adenineU-’4C]ADP-ribose-histone were synthesized by an NAD:arginine ADP-ribosyltransferase from [’*C]NAD and agmatine and histone, respectively. The pseudofirst order rate constants for breakdown of the two components either in 0.4 N NaOH or in 0.4 M neutral hydroxylamine were identical. Hydroxylamine treatment of [‘4C]ADP-ribose-agmatine or [32P]ADP-ribo...
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PARP catalysed ADP-ribosylation is a post-translational modification involved in several physiological and pathological processes, including cellular stress. In order to visualise both Poly-, and Mono-, ADP-ribosylation in vivo, we engineered specific fluorescent probes. Using them, we show that amino-acid starvation triggers an unprecedented display of mono-ADP-ribosylation that governs the fo...
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The modification of serines by molecules of ADP-ribose plays an important role in signaling that the DNA in a cell has been damaged and needs to be repaired.
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NAD+ glycohydrolase (NADase) and non-enzymic ADP-ribosylation have been thought to be involved in the regulation of mitochondrial Ca2+ fluxes. In this study it was found that several conditions (5 mM nicotinamide, 5 mM 3-aminobenzamide, 2 mM EDTA, 1 mM ATP, 10 mM dithiothreitol) known to strongly inhibit the NADase decreased ADP-ribosylation in bovine liver mitochondrial membranes with [32P]NAD...
متن کاملADP-ribosylation of nuclear proteins.
ADP-ribosylation can be defined as the postsynthetic modification of protein by the covalent attachment of the ADP-ribose moiety of NAD+. ADP-ribosylation of elongation-factor Tu is responsible for the inhibition of protein synthesis by both diptheria and Pseudomonas aeroginosa toxins (Hilz & Stone, 1976). The activation of membrane adenylate cyclase by cholera toxin is thought to occur by ADP-...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1985
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)36219-1